| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10550345 | Journal of Chromatography B | 2005 | 5 Pages |
Abstract
Tubulin, a potential target for anti-cancer drugs, has been purified in one step and obtained as flow-through fraction directly from an extract of a mammalian brain tissue by adsorption chromatography on H-CELBEADS, an indigenously developed rigid, superporous cross-linked cellulose based weakly hydrophobic adsorbent. The fibrous polymerized tubulin mass passed through the H-CELBEADS bed while the associated proteins were separated by adsorption. The final tubulin preparation was obtained free from other proteins as seen on SDS-PAGE. Purified tubulin was obtained in a yield of about 29Â mg/100Â g brain, and its bioactivity, evaluated through its ability to bind colchicine, was found to be preserved.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Annamma Anil, Reena Pandit, Madhavi Indap, Arvind Lali,