Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10550516 | Journal of Chromatography B | 2005 | 5 Pages |
Abstract
A sensitive and specific method was developed for quantification of alprazolam and its two metabolites 4-hydroxyalprazolam and α-hydroxyalprazolam in plasma. The work up procedure was solid phase extraction. Liquid chromatography-mass spectrometry (LC-MS) was used for separation, detection and quantification of the analytes. The limit of quantitation (LOQ) was 0.05 ng/mL for alprazolam and the two metabolites. The extraction recovery was more than 82% for alprazolam and its metabolites. The within- and between-assay coefficients of variation were in the range of 1.9-17.9%. The method was used for determination of the pharmacokinetics parameters of alprazolam and its two metabolites in healthy Caucasian subjects who ingested 1 mg of alprazolam.
Related Topics
Physical Sciences and Engineering
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Authors
Annika Allqvist, Agneta Wennerholm, Jan-Olof Svensson, Rajaa A. Mirghani,