Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10551021 | Journal of Chromatography B | 2005 | 6 Pages |
Abstract
We devised a sensitive and simple method to determine alpha-methyltryptamine (AMT) and 5-methoxy-N,N-diisopropyltryptamine (5MeO-DIPT) in whole blood and urine, using gas chromatography-mass spectrometry (GC-MS). AMT and 5MeO-DIPT were extracted using an Extrelut® column with an internal standard, bupivacaine, followed by derivatization with acetic anhydride. The derivatized extract was used for GC-MS analysis of EI-SIM mode. The calibration curves of AMT and 5MeO-DIPT were linear in the concentration range from 10 to 750Â ng/ml in both blood and urine samples. The method detection limit (MDL) of AMT and 5MeO-DIPT were 1Â ng/ml each in whole blood and 5Â ng/ml each in urine. This method should be most useful to accurately determine the presence of these drugs in blood and urine in clinical and forensic cases.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Tomomi Ishida, Keiko Kudo, Akiko Kiyoshima, Hiromasa Inoue, Akiko Tsuji, Noriaki Ikeda,