Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10551184 | Journal of Chromatography B | 2005 | 11 Pages |
Abstract
A novel solid-phase microextraction (SPME) method was developed for isolation of dextromethorphan (DM) and its main metabolite dextrorphan (DP) from human plasma followed by GC-MS determination. Three different polymers, poly(dimethylsiloxane) (PDMS), poly(ethylenepropyleneglycol) monobutyl ether (Ucon) and polyethylene glycol (PEG) were synthesized as coated fibers using sol-gel methodologies. DP was converted to its acetyl-derivative prior to extraction and subsequent determination. The porosity of coated fibers was examined by SEM technique. Effects of different parameters such as fiber coating type, extraction mode, agitation method, sample volume, extraction time, and desorption condition, were investigated and optimized. The method is rapid, simple, easy and inexpensive and offers high sensitivity and reproducibility. The limits of detection are 0.010 and 0.015 ng/ml for DM and DP, respectively. The precisions for both analytes are below 5% (n = 5). The correlation coefficient was satisfactory (r2 > 0.99) for both DM and DP. Linear ranges were obtained from 0.03 ng/ml to 2 μg/ml for DM and from 0.05 ng/ml to 2 μg/ml for DP.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Habib Bagheri, Ali Es-haghi, Mohammad-Reza Rouini,