| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10551196 | Journal of Chromatography B | 2005 | 5 Pages |
Abstract
Salvinorin A was quantitated in human and rhesus monkey plasma, rhesus monkey cerebrospinal fluid, and human urine by negative ion LC-MS/APCI. The method for Salvinorin A has been fully validated, the LLOQ using FDA guidelines is 2Â ng/mL for 0.5Â mL plasma samples. The linear range was from 2 to 1000Â ng/mL. Several derivatives in the Salvinorin family can also be analyzed by this method; d3-Salvinorin A was prepared and used as internal standard. The metabolite Salvinorin B can be semi quantitatively determined. The method has been used to establish that Salvinorin B is the principal metabolite of Salvinorin A ex vivo and to establish the analytical method to study in vivo samples.
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Authors
Mark S. Schmidt, Thomas E. Prisinzano, Kevin Tidgewell, Wayne Harding, Eduardo R. Butelman, Mary J. Kreek, Daryl J. Murry,