Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10552263 | Journal of Chromatography B | 2005 | 10 Pages |
Abstract
Methodology using MAE/SPME/GC-MS is being pursued for the analysis of organic pollutants in sebum. The microwave-assisted extraction (MAE) of standards of semi volatile organic pollutants from sebum was optimized. All compounds were extracted from sebum with recoveries analyzed by GC/MS ranging from 94% to 100% under the optimum MAE conditions: 10 mL acetone-hexane (2:1), 60 °C, and 10 min microwave heating. To improve the detection limits a SPME procedure was optimized. Linearity ranged from 0.70 ppb to 25 ppb. R.S.D. were in the range of 1-23% for the SPME step. Preliminary real samples were analyzed and a range of compounds was detected. The optimized MAE/SPME/GC-MS methodology promises to be useful for different applications.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Liz M. DÃaz-Vázquez, Oscar GarcÃa, Zorangelys Velázquez, Ivelisse Marrero, Osvaldo Rosario,