Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10552270 | Journal of Chromatography B | 2005 | 10 Pages |
Abstract
This paper describes two high-throughput liquid chromatography-tandem mass spectrometry (LC-MS-MS) methods for the screening of two important classes of drugs in equine sports, namely corticosteroids and basic drugs, at low ppb levels in horse urine. The method utilized a high efficiency reversed-phase LC column (3.3 cm L Ã 2.1 mm i.d. with 3 μm particles) to provide fast turnaround times. The overall turnaround time for the corticosteroid screen was 5 min and that for the basic drug screen was 8 min, inclusive of post-run and equilibration times. Method specificity was assessed by analysing a total of 35 negative post-race horse urine samples. No interference from the matrices at the expected retention times of the targeted masses was observed. Inter-day precision for the screening of 19 corticosteroids and 48 basic drugs were evaluated by replicate analyses (n = 10) of a spiked sample on 4 consecutive days. The results demonstrated that both methods have acceptable precision to be used on a routine basis. The performance of these two methods on real samples was demonstrated by their applications to drug administration and positive post-race urine samples.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Gary N.W. Leung, Evonne W. Chung, Emmie N.M. Ho, W.H. Kwok, David K.K. Leung, Francis P.W. Tang, Terence S.M. Wan, Nola H. Yu,