Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10552345 | Journal of Chromatography B | 2005 | 8 Pages |
Abstract
I, 2-{[5,7-dipropyl-3-(trifluoromethyl)-1,2-benzisoxazol-6-yl]oxy}-2-methyl propionic acid is an α peroxisome proliferator-activated receptor (PPAR) agonist with some γ activity being investigated for potential use in the treatment of Type II diabetes mellitus and dyslipidemia. Two automated liquid-liquid extraction methods were developed and validated for the determination of I in human plasma. Concentrations of I were determined over a wide range of clinical doses. For Method A, plasma was acidified and extracted with ethyl acetate using a fully automated procedure. Analysis was performed by LC-MS/MS with a turbo ionspray source in negative ion mode. For Method B, a larger volume of plasma was extracted and a heated nebulizer source was used on the mass spectrometer. Method A was linear from 0.05 to 50 ng/mL and Method B from 0.2 to 1000 ng/mL. Validation procedures showed that both methods were robust, specific and reproducible.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Kerry A. Riffel, Michelle A. Groff, Larissa Wenning, Hengchang Song, Man-Wai Lo,