Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10553702 | Journal of Pharmaceutical and Biomedical Analysis | 2005 | 6 Pages |
Abstract
A simple, rapid, sensitive and specific reversed-phase high performance liquid chromatographic method involving ultraviolet detection (HPLC-UV) was developed for analysis of didanosine in drug substance and formulated products, tablets. Chromatography was carried out on a pre-packed, Lichrospher 100 Rp-8 (5.0 μm, 250 mm Ã 4.0 mm) column using 0.01 M sodium acetate solution:methanol (85:15, v/v) adjusted to pH 6.5 with acetic acid as mobile phase at a flow rate of 1.5 ml/min and a 248 nm detection. Hypoxantine was confirmed as the main degradation product. The assay was linear over the concentration range of 50-150 μg/ml (R â 0.999). The method was validated for accuracy and precision.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Antonia Maria Cavalcanti de Oliveira, Teresa Cristina Raposo Löwen, Lúcio Mendes Cabral, Elizabeth Moreira dos Santos, Carlos Rangel Rodrigues, Helena C. Castro, Tereza Cristina dos Santos,