| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10553917 | Journal of Pharmaceutical and Biomedical Analysis | 2005 | 6 Pages |
Abstract
The concentration of low molecular weight compounds in tissues can yield valuable information about the metabolic state of an organism. Studies of changes in the metabolic state or metabonomics can reflect disease pathways, drug action, or toxicity. This research aims to develop a new approach, tissue targeted metabonomics. Microdialysis sampling and microcoil NMR analysis are employed to compare basal and ischemic metabolic states of various tissues (blood, brain, and heart) of Sprague-Dawley rats. Microdialysis sampling is localized, making the metabolic profile tissue specific. Coupling to NMR analysis is highly advantageous, because a complete metabolic profile is obtained in a single spectrum. However, small sample volumes and low analyte concentrations make analysis of microdialysis samples challenging. Microcoil NMR uses low sample volumes and has improved mass sensitivity, relative to standard 5Â mm probes. The coupling of these techniques is a potentially powerful tool for metabonomics analysis.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Kristin E. Price, Shannon S. Vandaveer, Craig E. Lunte, Cynthia K. Larive,