Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10553984 | Journal of Pharmaceutical and Biomedical Analysis | 2005 | 6 Pages |
Abstract
A method based on capillary zone electrophoresis (CZE) was used to study the interaction between low molecular weight heparin (LMWH) and interleukin 2 (IL-2). The results showed that the increase of the concentration of LMWH led to the decrease of the peak height and the increase of the peak width of IL-2, but the peak areas were kept constant. The binding constant of IL-2 with LMWH was calculated as 1.2Â ÃÂ 106Â Mâ1 by Scatchard analysis, which is in good agreement with the results found in the references using enzyme-linked immunosorbent assay (ELISA). The results demonstrated that the interaction between IL-2 and LMWH is of fast on-and-off kinetic binding reaction. CZE might be used to study not only slow on-and-off rates interactions, but also fast on-and-off rates ones. The binding constant can be calculated easily, and the method can be applied to study a wide range of heparin-protein interactions.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Aiye Liang, Xinya He, Yuguang Du, Keyi Wang, Yingsing Fung, Bingcheng Lin,