Article ID Journal Published Year Pages File Type
10554001 Journal of Pharmaceutical and Biomedical Analysis 2005 5 Pages PDF
Abstract
A sensitive liquid chromatographic method with electrochemistry (LC/EC) was developed for the determination of trace of minoxidil in hamster skin follicles after topical administration of the ear using various formulations. The minoxidil in the sebaceous glands of the hamster ear was isolated from the skin and the follicles in different skin layers were treated with aqueous trichloroacetic acid followed by acetonitrile. The supernatant was directly injected into the LC/EC system and minoxidil was detected by oxidation at +800 mV versus Ag/AgCl using a glassy carbon electrode. The analytical recoveries were between 94.4 and103.1% and the linearity was excellent up to 250 μg/ml with a regression coefficient (r2) of 0.9988. The LC/EC and the widely used radiolabeled scintillation methods agree well and both show high sensitivities. The LC/EC method is rapid and cost-effective with a detection limit of only 1 ng/ml.
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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