Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10554020 | Journal of Pharmaceutical and Biomedical Analysis | 2005 | 6 Pages |
Abstract
A method for the determination of local anaesthetics and their impurities - 2,6-dimethylaniline and o-toluidine - by high-performance liquid chromatographic method with amperometric detection has been developed. The analysis was performed in an isocratic mode on a reversed phase Luna column 5 μm C-18 (100 mm Ã 4.6 mm). A mobile phase [0.01 mol lâ1 Tris buffer of pH 7.9:acetonitrile (45:55)] was selected for the separation and determination of studied anaesthetics and their impurities. Chromatograms were recorded for 500 s by means of an amperometric detector at a potential of +1.0 V of the glassy carbon electrode versus the reference electrode Ag/AgCl. The proposed liquid chromatographic method was successfully applied to the analysis of commercially available pharmaceutical preparations. The limit of the detection for 2,6-dimethylaniline and o-toluidine was 0.8 ng mlâ1. The limit of qantitation, considering a signal to noise ratio was 1.5 ng mlâ1. The method developed in this study is sensitive and selective and can be applied to routine studies of pharmaceuticals in the form of cream and injection.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Zbigniew FijaÅek, Emil BaczyÅski, Agata PiwoÅska, MaÅgorzata Warowna-GrzeÅkiewicz,