| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10554108 | Journal of Pharmaceutical and Biomedical Analysis | 2005 | 6 Pages |
Abstract
A case study in identifying and eliminating the source of autosampler carryover in a bioanalytical HPLC-MS/MS assay is described. Through a series of systematic experiments, the carryover was traced to the injection valve and was eliminated by switching from a partial loop to a full loop injection, which provided more effective flushing of the sample flow path. The susceptibility of the HPLC system to carryover was demonstrated to depend on the absolute sensitivity of the detection method and the mass of analyte injected at the assay lower limit of quantitation (LLOQ).
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
P.T. Vallano, S.B. Shugarts, E.J. Woolf, B.K. Matuszewski,