Article ID Journal Published Year Pages File Type
10554149 Journal of Pharmaceutical and Biomedical Analysis 2005 6 Pages PDF
Abstract
A simple, accurate, precise and rapid HPLC method with UV detection has been validated in order to determine the in vitro transdermal absorption of sumatriptan succinate. The HPLC method is a modification of that described by Nozal et al. [M.J. Nozal, J.L. Bernal, L. Toribio, M.T. Martín, F.J. Diez, J. Pharm. Biomed. Anal. 30 (2002) 285-291]. Separation was carried out on a 250 mm Kromasil C18 column at room temperature. The detector response, at 282.7 nm, was found to be linear in a concentration range between 0.145 and 145 μM. The limit of detection (LOD) was 0.019 μM and the limit of quantification (LOQ) was 0.145 μM.
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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