Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10554149 | Journal of Pharmaceutical and Biomedical Analysis | 2005 | 6 Pages |
Abstract
A simple, accurate, precise and rapid HPLC method with UV detection has been validated in order to determine the in vitro transdermal absorption of sumatriptan succinate. The HPLC method is a modification of that described by Nozal et al. [M.J. Nozal, J.L. Bernal, L. Toribio, M.T. MartÃn, F.J. Diez, J. Pharm. Biomed. Anal. 30 (2002) 285-291]. Separation was carried out on a 250 mm Kromasil C18 column at room temperature. The detector response, at 282.7 nm, was found to be linear in a concentration range between 0.145 and 145 μM. The limit of detection (LOD) was 0.019 μM and the limit of quantification (LOQ) was 0.145 μM.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
A. FemenÃa-Font, V. Merino, V. Rodilla, A. López-Castellano,