Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10554346 | Journal of Pharmaceutical and Biomedical Analysis | 2005 | 7 Pages |
Abstract
The migration behaviour of acetaminophen and p-aminophenol was investigated by capillary electrophoresis. The influence of different parameters (pH, nature and concentration of the running buffer and applied voltage) on the migration time, peak symmetry, efficiency and resolution was systematically investigated. The two analytes can be well separated within 4 min in a 57 cm fused-silica capillary at a separation voltage of 18 kV in a 50 mM borate buffer adjusted to pH 9.5. Correlation coefficients for calibration curves in the range 0.2-200 μg mlâ1 for acetaminophen and 0.3-3 μg mlâ1 for p-aminophenol were higher than 0.999. The sensitivity of detection is 4.2 ng mlâ1 for acetaminophen and 11.2 ng mlâ1 for p-aminophenol. The method was applied to the analysis of various commercially available acetaminophen dosage forms with recoveries of 98.4-100.7%.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Tomás Pérez-Ruiz, Carmen MartÃnez-Lozano, Virginia Tomás, Raquel Galera,