Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10554348 | Journal of Pharmaceutical and Biomedical Analysis | 2005 | 7 Pages |
Abstract
A flow-injection chemiluminescence method for the determination of tryptophan was proposed, which was based on an intense chemiluminescence of tryptophan in hydrogen peroxide-nitrite-sulfuric acid medium. The chemiluminescence reaction was attributed to peroxidation and epoxidation of tryptophan by peroxynitrous acid, and subsequent decomposition of the formed dioxetane. The chemiluminescence intensity was linear with tryptophan in the range of 6.0 Ã 10â7 to 3.0 Ã 10â5 mol lâ1 and the limit of detection (S/N = 3) was 1.8 Ã 10â7 mol lâ1. The proposed method was applied to the analysis of tryptophan in pharmaceutical preparations and human serum.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Yao-Dong Liang, Jun-Feng Song,