Amperometric biosensor based on horseradish peroxidase for biogenic amine determinations in biological samples

An amperometric biosensor for total biogenic amine determinations, using a carbon paste electrode modified with horseradish peroxidase (HRP) enzyme is described. The HRP immobilization on graphite was made using bovine serum albumin, carbodiimide and glutaraldehyde. The biosensor response was optimized using serotonin and it presented the best performance in 0.1 mol l−1 phosphate buffer (pH = 7.0) containing 10 μmol l−1 of hydrogen peroxide. Under optimized operational conditions at −50 mV versus SCE, a linear response range from 40 to 470 ng ml−1 was obtained. The detection limit was 17 ng ml−1 and the response time was 0.5 s. The proposed sensor presented a stable response during 4 h under continuous monitoring. The difference of the response between six sensor preparations was <2%. The sensor was applied in the determination of total biogenic amines (neurotransmitters) in rat blood samples with success, obtaining a recovery average of 102%.