Proteomics and gene expression analyses of mitochondria from squalene-treated apoE-deficient mice identify short-chain specific acyl-CoA dehydrogenase changes associated with fatty liver amelioration

► Squalene (1 g/kg/day) decreased triglycerides in apoE-ko mice hepatocytes. ► Mitochondrial hepatic proteomic analysis unveiled 18 proteins modified by squalene. ► Squalene action executes a complex regulation of mitochondrial protein expressions. ► Squalene-induced decrease in steatosis is related to ACADS and it could be a biomarker. ► MAT1A is increased by squalene administration.