| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10556049 | Journal of Proteomics | 2012 | 12 Pages |
Abstract
⺠We have identified a potential plasma protein biomarker panel related to the intake of transfat. ⺠The human liver cell line HepG2-SF was used as a model system. ⺠The secretomes were analyzed by SILAC, DIGE and gene expression microarray analysis. ⺠We found 37 proteins that were regulated specifically in response to elaidic acid exposure.
Keywords
Difference in gel electrophoresisGene expression microarray analysisGEMAFFAHSATRFASRMAUChuman serum albuminOrbitrapelaidic acidFatty acidFree fatty acidTrans fatty acidTrans fatty acidsstable isotope labeling of amino acids in cell culturecardiovascular diseasePlasma biomarkerDIGECVDserum freeHepG2 cellsSILACCoefficient of varianceMass spectrometryarea under curveselected reaction monitoring
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Toke Peter Krogager, Lone Vendel Nielsen, Steffen Bak, Clifford Young, Carla Ferreri, Ole Nørregaard Jensen, Peter Højrup, Vladimiros Thoma, Ida B. Thøgersen, Jan J. Enghild,