Effects of nitric oxide synthase-3 overexpression on post-translational modifications and cell survival in HepG2 cells

Article ID	Journal	Published Year	Pages	File Type
10556057	Journal of Proteomics	2012	16 Pages	PDF

Abstract

âº NOS-3 overexpression decreased cell proliferation and increased cell death. âº NOS-3 overexpression increased oxidative/nitrosative stress. âº NOS-3 overexpression increased post-translational modifications. âº The protein folding system was profoundly disturbed in NOS-3 overexpressed cells. âº Cytoskeleton, metabolism, immune and antioxidant responses were also altered.

Keywords

GRP78 endoplasmin NOS-3 MDA MMTS PPAC PRDX6 Alpha enolase grp75 NDKB Enoyl-CoA hydratase atpB protein disulfide isomerase A3 PDIA3 N-nitro-l-arginine methyl ester ATP synthase subunit beta DTT GSSG GSH heat shock cognate 71 kDa protein DNPH 2,4-dinitrophenylhydrazine l-NAME ROS Oxidative stress dithiothreitol Actb lactate dehydrogenase LDH malondialdehyde methyl methanethiosulfonate tapasin Nitrosylation Nitration Nitric oxide Hepatocarcinoma 78 kDa glucose-regulated protein protein disulfide isomerase peroxiredoxin 6 calr reduced glutathione Carbonylation calreticulin Reactive oxygen species