| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10556178 | Journal of Proteomics | 2011 | 15 Pages |
Abstract
⺠Non-invasive sampling, modifed filter-aided sample preparation and MS analysis. ⺠Keratins, K1 and K10 were identified as major proteins of human skin. ⺠Most of the keratin methionine sites were partially oxidized in intact human skin. ⺠Met259, Met262, Met296, Met469 from K1 were the most susceptible sites to oxidation. ⺠Identified methionine residues as potential biomarkers of oxidative skin damage.
Keywords
FASPTFAPMFDTTDNPHDHBIAABHTMSRMALDI-TOFRCMTRISESISDS2-Amino-2-hydroxymethyl-1,3-propanediol2,4-dinitrophenylhydrazine2,5-dihydroxybenzoic acidMS/MSROSPeptide mass fingerprintingTrifluoroacetic acidUltraviolet radiationMethionine oxidationfilter-aided sample preparationstandard error of the meandithiothreitolmatrix-assisted laser desorption ionization-time of flightsodium dodecyl sulfateMass spectrometryTandem mass spectrometryStratum corneumMALDI-TOF/MSmethionine sulfoxide reductaseSEMEICbutylated hydroxytolueneliquid chromatographyextracted ion chromatogramReactive oxygen specieselectrospray ionizationiodoacetamide
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Seon Hwa Lee, Kohei Miyamoto, Takaaki Goto, Tomoyuki Oe,