Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10556179 | Journal of Proteomics | 2011 | 19 Pages |
Abstract
⺠Capture of the Fc receptor-cytoskeleton complex from the surface of live human primary cells. ⺠Random and independent sampling of parent and fragment ions by LC-ESI-MS/MS with log normal ion intensity values. ⺠Log intensity of parent and fragment ions compared across treatments, proteins and peptides by ANOVA and Tukey-Kramer. ⺠Type I error of protein identification calculated by Chi square comparison of peptide-to- protein ratio versus random spectra. ⺠Extended network of protein interactions specific to IgG-ligand calculated after subtracting proteins on control beads.
Keywords
PIK3s.e.GEFLARCAEBSFPMSFTRISPI3PRPMIRCFPBSNCBIESIPNMCIDGTPase Activating ProteinPMAMS/MSCollision-induced dissociationPhorbol esteractinTris(hydroxymethyl)aminomethanetubulinstandard errordaltondyneinGAPMass spectrometryTandem mass spectrometryGuanine exchange factorPhosphate buffered salinePhenylmethanesulfonyl fluorideNational Center for Biotechnology InformationMyosinrelative centrifugal forceKinesinFc receptorElectro spray ionization
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Angelica K. Florentinus, Andy Jankowski, Veronika Petrenko, Peter Bowden, John G. Marshall,