Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10556224 | Journal of Proteomics | 2011 | 6 Pages |
Abstract
⺠Thou shalt not add 150 mM salt to the sample. ⺠Thou shalt capture at three pH values. ⺠Thou shalt not elute in 4 M urea, 1% CHAPS. ⺠Thou shalt not elute in 8 M urea, 2% CHAPS, 5% acetic acid. ⺠Thou shalt elute in boiling 4% SDS, 25 mM DTT (A Pentalogue found in proximity of Mount Sinai).
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Francesco Di Girolamo, Egisto Boschetti, Maxey C.M. Chung, Fiorella Guadagni, Pier Giorgio Righetti,