Saccharomyces cerevisiae in the stationary phase as a model organism - characterization at cellular and proteome level

► S. cerevisiae in the stationary phase was characterized at the cellular and proteome level. ► At the cellular level, optical density, cell viability, glycogen content, intracellular oxidation and cell energy metabolic activity were measured, while at the proteome level, protein profiles were analyzed using two-dimensional electrophoresis. ► The data obtained at both levels provide better insight into quiescence program state, which still remains poorly understood. ► Optimal time period reflecting a stable metabolic and oxidative state of the yeast was determined (2 to 8 days in PBS buffer-plateau 1). ► As such, this yeast system shows potential for studying changes in cell oxidant status as well as for energy metabolic activity in response to different environmental stressors.