A proteomics strategy for determining the synthesis and degradation rates of individual proteins in fish

► Protein concentration is defined by the relative rates of protein synthesis and degradation. ► MS-based proteomics method to determine the synthesis and degradation rates of individual proteins in tissues of fish. ► Can differentiate the rates of synthesis of homologous isoforms of parvalbumin in skeletal muscle from common carp. ► Experimental approach can be employed to determine the turnover of multiple proteins from other tissues in fish species.