| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10556330 | Journal of Proteomics | 2011 | 14 Pages |
Abstract
⺠Sub-fractionation of nasal epithelial cells (NEC) to improve the detection of low abundance proteins. ⺠Global proteome characterization based on a gel-free SCX-RPLC- chromatography coupled with MS/MS approach. ⺠Characterization of cell membrane proteins using a methanol-based solubilization before tryptic digestion. ⺠Pathway Analysis to profile molecular and cellular functions overrepresented in nasal epithelium. ⺠Extensive comparison of our data with previously published studies focusing on respiratory tract epithelium.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Tânia Simões, Nuno Charro, Josip Blonder, Daniel Faria, Francisco M. Couto, King C. Chan, Timothy Waybright, Haleem J. Isaaq, Timothy D. Veenstra, Deborah Penque,