Comparison of blood serum peptide enrichment methods by Tricine SDS-PAGE and mass spectrometry

► Three methods for reduction of serum abundant proteins with enrichment of small proteins and peptides were compared. ► Specifically, Differential Solubilisation (DS), Ultrafiltration, and Bio-Rad ProteoMiner Beads treatment were evaluated. ► DS allowed the identification of the highest number of peptides, enabling also the quantitative comparison of samples. ► Although with less efficiency compared to proteins, ProteoMiner Beads performed well in peptide enrichment. ► Ultrafiltration was probably affected by filter cloggings and requires higher sample amounts.