| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10556420 | Journal of Proteomics | 2012 | 13 Pages |
Abstract
⺠TiSH - an enrichment and fractionation strategy combining TiO2, SIMAC, and HILIC. ⺠TiO2 pre-enrichment ensures highly specific phosphopeptide enrichment efficiency. ⺠The SIMAC fractionation facilitates better recovery of multiphosphorylated peptides. ⺠The highly organic buffers in the strategy reduce absorptive losses of the pure phosphopeptides. ⺠6600 phosphopeptides are identified from 300 μg in a quantitative dimethyl experiment.
Keywords
NF-κBIPAIFN-γIKKFWHMLTQDHBHCDTFAdsRNAIMACppmACNDTTSDSFDRPBSJnkc-Jun N-terminal kinaseIκB kinaseJAK/STATMAPKdouble stranded RNAAdenosine TriphosphateATPAcetonitrilescxTrifluoroacetic acidinterferon-γTEABtriethylammonium bicarbonatestrong cation exchangeIngenuity Pathway AnalysisFractionationTiO2dithiothreitolsodium dodecyl sulfateMass spectrometryfull width at half maximumPhosphopeptide enrichmentreversed phasenuclear factor kappa-light-chain-enhancer of activated B cellsPhosphate buffered salineProtein phosphorylationPhosphoproteomicsparts-per-millionfalse discovery rateHILICmitogen-activated protein kinaseliquid chromatographyhydrophilic interaction liquid chromatographyhigh-performance liquid chromatographyHPLCimmobilized metal affinity chromatography
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Kasper Engholm-Keller, Pernille Birck, Joachim Størling, Flemming Pociot, Thomas Mandrup-Poulsen, Martin R. Larsen,