| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10556475 | Journal of Proteomics | 2012 | 10 Pages |
Abstract
⺠The release of biotin-labeled proteins and molecular partners from streptavidin beads is a challenge. ⺠We described an on-bead digestion procedure as an alternative of elution protocols. ⺠This procedure allowed the identification of more proteins than others elution protocols. ⺠The identification of released proteins was carried out by mass spectrometry and validated by a functional assay.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Hidehiro Fukuyama, Sega Ndiaye, Jules Hoffmann, Jean Rossier, Sophie Liuu, Joëlle Vinh, Yann Verdier,