Article ID Journal Published Year Pages File Type
10556475 Journal of Proteomics 2012 10 Pages PDF
Abstract
► The release of biotin-labeled proteins and molecular partners from streptavidin beads is a challenge. ► We described an on-bead digestion procedure as an alternative of elution protocols. ► This procedure allowed the identification of more proteins than others elution protocols. ► The identification of released proteins was carried out by mass spectrometry and validated by a functional assay.
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
Authors
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