| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10556510 | Journal of Proteomics | 2012 | 10 Pages |
Abstract
⺠LY294002 inhibits the PI3K pathway, often activated in colorectal cancer. ⺠Cytosolic, nuclear and mitochondrial proteomes were analysed using iTRAQ. ⺠6 Aminoacyl-tRNA synthetases reduced with no apparent effect on global protein synthesis. ⺠Shuttling of p53 from the nucleus to the cytosol. ⺠LY294002 decreases several chaperones and glycolytic proteins.
Keywords
FCSPI3KECLESIIEFLY294002IC50PKBHRPPMFITRAQDMSOIPGimmobilized pH gradientLC–MS/MSPI3 kinasehorse-radish peroxidasescxSDS-PAGESodium dodecyl sulfate polyacrylamide gel electrophoresisTEABenhanced chemiluminescencetriethylammonium bicarbonatestrong cation exchangeisoelectric focusingDimethyl sulfoxideColorectal cancerfetal calf serumliquid chromatography–mass spectrometryphosphatase and tensin homologue deleted on chromosome 10phosphoinositide 3-kinasehalf maximal inhibitory concentrationprotein kinase BPtenCRCelectrospray ionization
Related Topics
Physical Sciences and Engineering
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Authors
Duthika M. Mallawaaratchy, Swetlana Mactier, Kimberley L. Kaufman, Katherine Blomfield, Richard I. Christopherson,