Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10557170 | Microchemical Journal | 2005 | 8 Pages |
Abstract
This work presents an electroanalytical methodology developed for square-wave voltammetry based in the electrochemical reduction in hanging mercury drop electrode (HMDE), which is simple, fast, reliable and sensitive for determination of moxifloxacin (MOXI) in tablets and spiked urine human samples. The support electrolyte that provided a more defined and intense peak current for MOXI determination was the phosphate buffer 0.04 mol lâ 1 pH 8.0. In the best-optimized conditions the drug presented an only peak of reduction at â 1.38 V vs. Ag/AgCl, using an Eacc. of â 0.30 V. An LOD of 0.44 and 3.20 ng mlâ 1 and an LOQ of 1.46 and 10.60 ng mlâ 1 were found for the pure standard of moxifloxacin and in the presence of matrix, respectively. A good recovery was obtained for assay spiked urine samples and a good quantification of MOXI was achieved in a commercial formulation. The methodology proposed was more sensitive than the spectrofluorimetric and spectrophotometric method with precision and accuracy equivalent.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Magno Aparecido G. Trindade, Glaucia Maria da Silva, Valdir Souza Ferreira,