Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10557386 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2005 | 5 Pages |
Abstract
An integrated microchip was presented for selective recognition of Z-l-Phe-OH-NBD, using molecular imprinting technique. Molecularly imprinted polymer (MIP) were prepared by copolymerization in the presence of template molecule Z-l-Phe-OH-NBD, in which methacrylic acid and 4-vinylpyridine were used as functional monomers and ethylene dimethacrylate used as crosslinker. Imprinted polymer particles were introduced into a microchannel fabricated with a new material i.e. poly(methylvinylsiloxane) by simply rapid prototyping method. Imprinted effects were evaluated by laser-induced fluorescence (LIF) detection where the results indicated that good selective recognition for Z-l-Phe-OH-NBD in the imprinted polymer was obtained; the adsorption percentage of Z-l-Phe-OH-NBD was 61%. In contrast to conventional molecular imprinting analysis, integration shortened overall analysis time from 4Â h to 10Â min.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Jian-Du Lei, Ai-Jun Tong,