Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10557503 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2005 | 6 Pages |
Abstract
A new resonance light scattering (RLS) assay of protein is presented. In Tris-NaOH (pH = 10.93) buffer, the RLS of rutin-cetylpyridine bromide (CPB) system can be greatly enhanced by protein, including bovine serum albumin (BSA) and human serum albumin (HSA). The enhanced RLS intensities are in proportion to the concentration of proteins in the range of 5 à 10â9 to 2.5 à 10â6 g mlâ1 for BSA and 2.5 à 10â8 to 3.5 à 10â6 g mlâ1 for HSA. The detection limits (S/N = 3) are 3.0 ng mlâ1 for BSA and 10.0 ng mlâ1 for HSA. Samples are determined satisfactorily.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Yang Liu, Jinghe Yang, Shufang Liu, Xia Wu, Benyu Su, Tao Wu,