Article ID Journal Published Year Pages File Type
10557583 Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2005 6 Pages PDF
Abstract
Based on the enhancement of the resonance light scattering (RLS) of Congo Red (CR) by nucleic acid, a new quantitative method for nucleic acid is developed. In the Tris-HCl buffer (pH 10.5), the weak light scattering of CR is greatly enhanced by addition of nucleic acid and CTMAB, the maximum peak is at 560 nm and the enhanced intensity of RLS is in proportion to the concentration of nucleic acid. The linear range is 1.0×10−9 to 1.0×10−6 g ml−1, 7.5×10−8 to 1.0×10−6 g ml−1 and 7.5×10−8 to 2.5×10−6 g ml−1 for herring sperm DNA, calf thymus DNA and yeast RNA, and the detection limits are 0.019, 0.89 and 1.2 ng ml−1 (S/N = 3), respectively. Actual biological samples were satisfactorily determined.
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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