Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10557873 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy | 2005 | 5 Pages |
Abstract
The property of carvedilol in acid-alkaline medium was studied by spectrofluorimetry. Effects of organic solvents on carvedilol fluorescence spectra were examined and the reason was discussed. A simple, rapid and high sensitive fluorimetric method for the determination of carvedilol in medicine is developed. The measurement of relative fluorescence intensity was carried out at 356 nm with excitation at 254 nm. Effects of pH, standing time and foreign ions on the determination of carvedilol have been examined. A linear relationship was obtained between the relative fluorescence intensity and concentration of carvedilol in the range of 0.50-270 ng mlâ1. The linear regression equation of the calibration graph is C = 0.000543F â 0.002 (C is concentration (μg mlâ1) and F is relative fluorescence intensity in the equation), with a correlation coefficient of linear regression of 0.9998 and relative standard deviation of 2.31%. The detection limit of this method is 0.19 ng mlâ1, and recovery is from 98.70 to 102.1%. This method can be used for determination of carvedilol in tablet. The results obtained by this method agreed with those by the official method.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Li Xiao Xu, Ni Hui, Li Ying Ma, Huai You Wang,