Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10559463 | Talanta | 2011 | 6 Pages |
Abstract
A novel piezoelectric (PZ) immunosensor for the direct detection of malarial Plasmodium falciparum histidine rich protein-2 (PfHRP-2) antigen was developed. The mixed self-assembled monolayers (SAMs) of thioctic acid and 1-dodecanethiol were formed on gold surface of quartz crystal. Cyclic voltammetry, electrochemical impedance spectroscopy and surface Raman spectroscopy techniques were used to characterize the mixed SAMs. The rabbit anti-PfHRP-2 antibodies were coupled on mixed SAM modified gold surface of quartz crystal via NHS/EDC activation method. The PZ immunosensor was applied to detect PfHRP-2 in the linear range of 15-60Â ng/ml with a detection limit of 12Â ng/ml. It was also found that even after 14 days of storage, 50% of the activity still remained. Clinical human serum samples were tested with this method, and the results were in agreement with those obtained from commercially available ICT kit (NOW® Malaria).
Related Topics
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Authors
M.K. Sharma, V.K. Rao, S. Merwyn, G.S. Agarwal, Sanjay Upadhyay, R. Vijayaraghavan,