Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10559505 | Talanta | 2011 | 8 Pages |
Abstract
A novel competitive electrochemical cytosensor was reported by using aptamer (Apt)-quantum dots (Qdots) conjugates as a platform for tumor cell recognition and detection. The complementary DNA (cDNA), aptamer and Qdots could be assembled to the gold electrode surface. When the target cells existed, they could compete with cDNA to bind with Apt-Qdots conjugates based on the specific recognition of aptamer to MUC1 protein overexpressed on the cell surface, which resulted in the denaturation of double-stranded DNA structure and the release of the Apt-Qdots conjugates from the electrode. Electrochemical stripping measurement was then employed to determine the Cd2+ concentration in Qdots left at the electrode. The peak current was inversely proportional to the logarithmic value of cell concentration ranging from 1.0 Ã 102 to 1.0 Ã 106 cells mLâ1 with a detection limit of 100 cells mLâ1. Meanwhile, the recognition of aptamer to the target cells could be clearly observed through the strong fluorescence from Qdots. This is an example of the combination of aptamer and nanoparticles for the application of cell analysis, which is essential to cancer diagnosis and therapy.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Jingjing Li, Mi Xu, Haiping Huang, Jinjun Zhou, E.S. Abdel-Halimb, Jian-Rong Zhang, Jun-Jie Zhu,