Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10559511 | Talanta | 2011 | 5 Pages |
Abstract
This work developed a novel method to greatly enhance the electrochemiluminescence (ECL) of CdS quantum dots (QDs). The ECL amplification was achieved by the assembly of QDs on poly (diallyldimethylammonium chloride)-functionalized carbon nanospheres (PFCNSs), and successfully employed for sensitive ECL biosensing of oxidase substrates. The carbon nanospheres were prepared by a “green” method, and the high loading of QDs on carbon nanospheres led to a 4-times increased ECL intensity with dissolved O2 as the coreactant. Using xanthine oxidase (XOD) as a model, an ECL biosensor was fabricated by immobilizing the enzyme on the mixing membrane of PFCNSs and QDs. The ECL biosensor showed a fast response to hypoxanthine with a linear concentration range from 2.5Â ÃÂ 10â8 to 1.4Â ÃÂ 10â5Â M. The limit of detection was 5Â nM at a signal-to-noise ratio of 3. The assay results of hypoxanthine in fish samples were in a good agreement with the reference values by amperometric technique. This facile approach to prepare the PFCNSs/QDs system for ECL biosensing could be of promising application in bioanalysis and electronic device.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Yangyang Zhang, Shengyuan Deng, Jianping Lei, Qiunan Xu, Huangxian Ju,