Article ID Journal Published Year Pages File Type
10559536 Talanta 2011 5 Pages PDF
Abstract
A simple and versatile approach for covalent immobilization of redox protein on solid surface via self-assembled technique and click chemistry is reported. The alkynyl-terminated monolayers are obtained by self-assembled technique, then, azido-horseradish peroxidase (azido-HRP) was covalent immobilized onto the formed monolayers by click reaction. The modified process is characterized by reflection absorption infrared spectroscopy (RAIR), surface-enhanced Raman scattering spectroscopy (SERS) and electrochemical methods. All the experimental results suggest that HRP is immobilized onto the electrode surface successfully without denaturation. Furthermore, the immobilized HRP shows electrocatalytic reduction for H2O2, and the linear range is from 5.0 to 700 μM. The heterogeneous electron transfer rate constant ks is 1.11 s−1 and the apparent Michaelis-Menten constant is calculated to be 0.196 mM.
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
Authors
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