Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10559555 | Talanta | 2011 | 6 Pages |
Abstract
A simple, highly sensitive, and label-free electrochemical impedance spectroscopy (EIS) aptasensor based on an anti-lysozyme-aptamer as a molecular recognition element, was developed for the detection of lysozyme. Improvement in sensitivity was achieved by utilizing gold nanoparticles (AuNPs), which were electrodeposited onto the surface of a gold electrode, as a platform for immobilization of the aptamer. To quantify the amount of lysozyme, changes in the interfacial electron transfer resistance (Ret) of the aptasensor were monitored using the redox couple of an [Fe(CN)6]3â/4â probe. The Ret increased with lysozyme concentration. The plot of Ret against the logarithm of lysozyme concentration is linear over the range from 0.1Â pM to 500Â pM with a detection limit of 0.01Â pM. The aptasensor also showed good selectivity for lysozyme without being affected by the presence of other proteins.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Zhengbo Chen, Lidong Li, Hongtao Zhao, Lin Guo, Xiaojiao Mu,