Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10559557 | Talanta | 2011 | 6 Pages |
Abstract
A novel affinity purification method for lysozyme (LZM) based on functionalized magnetic microspheres was developed. Tris(hydroxymethyl)aminomethane (Tris)-modified magnetic microspheres with specific affinity toward LZM were prepared using Tris as ligand and silica-coated magnetic microshperes as support. Transmission electron microscopy and magnetic property measurement results showed that the Tris-modified magnetic microspheres have a very good core-shell structure and high magnetization.The maximum binding capacity of LZM was about 108.6Â mg/g magnetic microspheres. LZM purified from chicken egg white had high purity and well-maintained activity of 8140Â U/mg. This magnetic-mediated LZM purification strategy has advantages of high efficiency, low cost and easy operation.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Guoqing Zhang, Qing Cao, Na Li, Kean Li, Feng Liu,