Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10559558 | Talanta | 2011 | 9 Pages |
Abstract
In this study, known impurities were traced in the LC-UV methods and new impurities present in polymyxin B and colistin bulk samples were characterized. To achieve this, each peak from the non-volatile system was collected separately and reinjected into an LC system with a volatile mobile phase coupled to MS. This way, collected impurity peaks were rechromatographed on a reversed phase column in order to separate the analyte from the buffer salts. Using this method, out of 39 peaks, five novel related substances were characterized in a polymyxin B bulk sample. Fourteen impurities, which were already reported in the literature were traced as good as possible in the LC-UV method. In the case of colistin, a total of 36 peaks were investigated, among which four new compounds. Additionally, 30 known impurities were traced in the LC-UV method.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Larissa Van den Bossche, Ann Van Schepdael, Shruti Chopra, Jos Hoogmartens, Erwin Adams,