Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10559566 | Talanta | 2011 | 6 Pages |
Abstract
In this study a double-bead sandwich assay, employing magnetic nanoparticles and gold nanoparticles is proposed. The magnetic nanoparticles allow specific capturing of the analyte in biological samples, while the optical properties of the gold nanoparticles provide the signal transduction. We demonstrated that a major improvement in the assay sensitivity was obtained by selecting an optimal gold nanoparticle size (60Â nm). A detection limit of 5-8Â ng/mL, a sensitivity of 0.6-0.8Â (pg/mL)â1 and a dynamic range of 3 orders of magnitude were achieved without any further amplification using the detection of prostate specific antigen in serum as a model system. The proposed assay has the ability to be easily implemented within a microfluidic device for point-of-care applications whereby the readout can be executed by a fast and cheap optical measurement.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Hilde Jans, Karolien Jans, Pieter-Jan Demeyer, Karel Knez, Tim Stakenborg, Guido Maes, Liesbet Lagae,