Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10559599 | Talanta | 2009 | 5 Pages |
Abstract
In this protocol, a fluorescent aptasensor based on magnetic separation for simultaneous detection thrombin and lysozyme was proposed. Firstly, one of the anti-thrombin aptamer and the anti-lysozyme aptamer were individually immobilized onto magnetic nanoparticles, acting as the protein captor. The other anti-thrombin aptamer was labeled with rhodamine B and the anti-lysozyme aptamer was labeled with fluorescein, employing as the protein report. By applying the sandwich detection strategy, the fluorescence response at 515Â nm and 578Â nm were respectively corresponding to lysozyme and thrombin with high selectivity and sensitivities. The fluorescence intensity was individually linear with the concentration of thrombin and lysozyme in the range of 0.13-4Â nM and 0.56-12.3Â nM, and the detection limits were 0.06Â nM of thrombin and 0.2Â nM of lysozyme, respectively. The preliminary study on simultaneous detection of thrombin and lysozyme in real plasma samples was also performed. It shows that the proposed approach has the good character for simultaneous multiple protein detection.
Related Topics
Physical Sciences and Engineering
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Analytical Chemistry
Authors
Liqing Wang, Lanying Li, Ying Xu, Guifang Cheng, Pingang He, Yuzhi Fang,