Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10559610 | Talanta | 2009 | 6 Pages |
Abstract
Disodium cromoglycate (SCG) is an anti-allergic drug, which is applied locally or inhaled. After administration, a very small portion of the drug is absorbed, being the most eliminated part unchanged in the urine and bile; therefore, its determination in urine is indicative of the dose absorbed. Here, the first spectroscopic method for the determination of SCG, making use of a sequential injection optosensor with terbium-sensitized luminescence detection, is described. The cationic resin Chelex-100 was used as solid support in the detection area. The measurements were made at 336/545 nm (λex/λem) and the system was calibrated for two sample volumes, 150 and 800 μl, depending on the samples analyzed. A detection limit of 15 ng mlâ1 and a RSD lower than 2% (n = 10) were observed using the highest sample volume. The proposed method does not use any organic solvent or surfactant, so being environmental friendly. The analyte was satisfactorily determined in pharmaceuticals and human urine, the latter being spiked at the concentrations found after the administration of the drug.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
L. Molina-GarcÃa, E.J. Llorent-MartÃnez, M.L. Fernández-de Córdova, A. Ruiz-Medina,