Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10559722 | Talanta | 2011 | 5 Pages |
Abstract
A sensitive method based on quantum dot (QD)-enhanced capillary electrophoresis-chemiluminescence (CE-CL) detection was developed for simultaneous determination of dopamine (DA) and epinephrine (E). In this work, CdTe QD was added into the running buffer of CE to catalyze the post-column CL reaction between luminol and hydrogen peroxide, achieving higher CL emission. Negative peaks were produced due to the inhibitory effects on CL emission from DA and E eluted from the electrophoretic capillary. The decrease in CL intensity was proportional to the concentration of DA and E in the range of 8.0Â ÃÂ 10â8-5.0Â ÃÂ 10â6Â M and 4.0Â ÃÂ 10â8-5.0Â ÃÂ 10â6Â M, respectively. Detection limits for DA and E were 2.3Â ÃÂ 10â8Â M and 9.3Â ÃÂ 10â9Â M, respectively. Using this method, the levels of DA and E in human urine from healthy donors were determined.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Yunsha Zhao, Shulin Zhao, Junming Huang, Fanggui Ye,