Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10559741 | Talanta | 2011 | 6 Pages |
Abstract
In this paper, a polydopamine (PDA) film is electropolymerized on the surface of bilayer lipid membrane (BLM) which is immobilized with horseradish peroxidase (HRP). The coverage of the PDA film on HRP/BLM electrode is monitored by electrochemical impedance spectroscopy (EIS). The electrocatalytic reduction of H2O2 at the PDA/HRP/BLM electrode is studied by means of cyclic voltammetry (CV). The biosensor has a fast response to H2O2 of less than 5 s and an excellent linear relationship is obtained in the concentration range from 2.5 Ã 10â7 to 3.1 Ã 10â3 mol Lâ1, with a detection limit of 1.0 Ã 10â7 mol Lâ1 (S/N = 3). The response current of BLM/HRP/PDA biosensor retains 84% of its original response after being stored in 0.1 mol Lâ1 pH 7.0 PBS at 4 °C for 3 weeks. The selectivity, repeatability, and storage stability of PDA/HRP/BLM biosensor are greatly enhanced by the coverage of polydopamine film on BLM.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Longzhen Zheng, Leyan Xiong, Dan Zheng, Yindi Li, Qiang Liu, Kui Han, Wen Liu, Kun Tao, Shaoming Yang, Jian Xia,