Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10559756 | Talanta | 2011 | 6 Pages |
Abstract
This study presents the investigation of bioconjugating ability of near-infrared (NIR) CdSeTe/ZnS quantum dots (QDs) (710Â nm) and visible CdSe QDs (595Â nm) in immunofluorescent staining for cancer biomarkers in gastric cancer tissues probed with the homemade Hadamard transform (HT) spectral imaging microscope and a commercial multispectral imaging system. The results show that imunostaining ability of NIR QDs probes is stronger than that of visible QDs when the two kinds of QDs are simultaneously used to probe the cancer biomarkers such as cytokeratin 20 (CK20) and proliferating cell nuclear antigen (PCNA) in gastric cancer tissues. Moreover, when the two QDs probes are used for immunostaining successively for the same target molecules, staining order has great influences on the final results due to their different conjugating ability to the marker proteins. The results imply that NIR QDs hold more promise for real-time imaging of tumor tissues due to its higher sensitivity and contrast. In addition, the results also demonstrate the potential of Hadamard transform spectral imaging as a useful tool in biomedical analysis and quantitative evaluation for tumor tissues.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Yue He, Hao Xu, Chuang Chen, Jun Peng, Hongwu Tang, Zhiling Zhang, Yan Li, Daiwen Pang,