Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10559965 | Talanta | 2005 | 5 Pages |
Abstract
A rapid and sensitive high-performance liquid chromatographic method has been developed for the simultaneous determination of the antidepressant fluoxetine and its active metabolite norfluoxetine in human plasma using paroxetine as internal standard. After liquid-liquid extraction, the compounds were separated on a C18 column using as mobile phase acetonitrile and 40Â mM potassium dihydrogen phosphate buffer (pH 2.3) in the ratio 31:69 (v/v). The quantification of fluoxetine and norfluoxetine was made by fluorescence detection at Ex/Em 230/312Â nm. The assay for each analyte was linear over the ranges 1-39 and 0.9-36Â ng/ml, respectively. For both compounds intra- and inter-day accuracy and precision ranged between â7.9-12.4 and 0.7-14.7%, respectively. The method was applied to the analysis of plasma samples obtained from healthy subjects treated with one single oral dose of 40Â mg fluoxetine.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Laurian Vlase, Silvia Imre, Sorin Leucuta,